LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1943..2039) /label=attL2 /note="attL2 (c)" misc_signal complement(2054..2073) /note="T7 Promoter/priming site (c)" primer_bind 2081..2097 /note="M13 Reverse priming site" gene 2210..3019 /note="Kanamycin resistance gene" rep_origin 3140..3813 /note="pUC origin" PCR_frame 669..1943 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..1963) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..1960) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(1951..3816,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 1921..1941 /note="TEV site" source 673..1920 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:22677 IMAGE:4698763" /tissue_type="Testis, embryonal carcinoma" /clone_lib="NIH_MGC_61" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 673..1920 /gene="ALG2" /gene_synonym=CDGIi /gene_synonym=hALPG2 /db_xref="GeneID:85365" /db_xref="HGNC:23159" /db_xref="MIM:607905" CDS 673..1920 /dnas_title="asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae)" /gene="ALG2" /gene_synonym=CDGIi /gene_synonym=hALPG2 /codon_start=1 /product="asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae)" /protein_id="AAH17876.1" /db_xref="GI:17389715" /db_xref="GeneID:85365" /db_xref="HGNC:23159" /db_xref="MIM:607905" /translation="MAEEQGRERDSVPKPSVLFLHPDLGVGGAERLVLDAALALQARG CSVKIWTAHYDPGHCFAESRELPVRCAGDWLPRGLGWGGRGAAVCAYVRMVFLALYVL FLADEEFDVVVCDQVSACIPVFRLARRRKKILFYCHFPDLLLTKRDSFLKRLYRAPID WIEEYTTGMADCILVNSQFTAAVFKETFKSLSHIDPDVLYPSLNVTSFDSVVPEKLDD LVPKGKKFLLLSINRYERKKNLTLALEALVQLRGRLTSQDWERVHLIVAGGYDERVLE NVEHYQELKKMVQQSDLGQYVTFLRSFSDKQKISLLHSCTCVLYTPSNEHFGIVPLEA MYMQCPVIAVNSGGPLESIDHSVTGFLCEPDPVHFSEAIEKFIREPSLKATMGLAGRA RVKEKFSPEAFTEQLYRYVTKLLV" misc_feature 673..1920 /note="ALG2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcgga ggagcagggc cgggaacggg actcggttcc caagccgtcg 721 gtgctgttcc tccacccaga cctgggcgtg ggcggcgctg agcggctggt gttggacgcg 781 gcgctggcgc tgcaggcgcg cgggtgtagc gtgaagatct ggacagcgca ctacgacccg 841 ggccactgtt tcgccgagag ccgcgagcta ccggtgcgct gtgccgggga ctggctgccg 901 cgaggcctgg gctggggcgg ccgcggcgcc gccgtctgcg cctacgtgcg catggttttc 961 ctggcgctct acgtgctgtt cctcgccgac gaggagttcg acgtggtagt gtgcgaccag 1021 gtgtctgcct gtatcccagt gttcaggctg gctagacggc ggaagaagat cctattttac 1081 tgtcacttcc cagatctgct tctcaccaag agagattctt ttcttaaacg actatacagg 1141 gccccaattg actggataga ggaatacacc acaggcatgg cagactgcat cttagtcaac 1201 agccagttca cagctgctgt ttttaaggaa acattcaagt ccctgtctca catagaccct 1261 gatgtcctct atccatctct aaatgtcacc agctttgact cagttgttcc tgaaaagctg 1321 gatgacctag tccccaaggg gaaaaaattc ctgctgctct ccatcaacag atacgaaagg 1381 aagaaaaatc tgactttggc actggaagcc ctagtacagc tgcgtggaag attgacatcc 1441 caagattggg agagggttca tctgatcgtg gcaggtggtt atgacgagag agtcctggag 1501 aatgtggaac attatcagga attgaagaaa atggtccaac agtccgacct tggccagtat 1561 gtgaccttct tgaggtcttt ctcagacaaa cagaaaatct ccctcctcca cagctgcacg 1621 tgtgtgcttt acacaccaag caatgagcac tttggcattg tccctctgga agccatgtac 1681 atgcagtgcc cagtcattgc tgttaattcg ggtggaccct tggagtccat tgaccacagt 1741 gtcacagggt ttctgtgtga gcctgacccg gtgcacttct cagaagcaat agaaaagttc 1801 atccgtgaac cttccttaaa agccaccatg ggcctggctg gaagagccag agtgaaggaa 1861 aaattttccc ctgaagcatt tacagaacag ctctaccgat atgttaccaa actgctggta 1921 gaaaacttgt actttcaagg ctCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 1981 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 2041 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 2101 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2161 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2221 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2281 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2341 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2401 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2461 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2521 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2581 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 2641 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 2701 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 2761 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 2821 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 2881 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 2941 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 3001 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 3061 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3121 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3181 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3241 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3301 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3361 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3421 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3481 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3541 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3601 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 3661 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 3721 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 3781 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //